Revising our view of the mammalian trascriptome
Brendan J. Frey University of Toronto and Canadian Institute for Advanced Research In the past 10 years, researchers have been trying to get a clear picture of what the library of expressed protein-coding and non-protein-coding mammalian genes looks like, and what the functions of these gene variants are. In this talk, I'll describe how we've used DNA microarrays and machine learning techniques to obtain new biological results on protein-coding and non-protein-coding genes. Our results reveal the existence of thousands of putative new protein-coding regions, explain discrepancies between existing gene libraries, and appear to be in conflict with recent results obtained by others. Taking a closer look at our revised view of the mammalian transcriptome, we've explored the global impact of alternative splicing and how it introduces functional diversity, and the question of how non-protein-coding genes, in particular microRNAs, act to regulate genes. References: Huang et al., RECOMB, 2006; Pan et al., Genes & Development 20, 2006; Frey et al., Nature Genetics 37:9, 2005; Pan et al., Molecular Cell 16, 2004.